Gene Cloning, Prokaryotic Expression and Functional Evaluation of Intimin from Enterohemorrhagic Escherichia Coli O157: H7 肠出血性大肠埃希菌O157:H7紧密黏附素的基因克隆、表达及功能初步研究
The fusion proteins composed of the main protective antigens intimin and Shiga-toxin of EHEC O157 ∶ H7 were expressed and purified. 以基因重组技术构建工程菌株表达肠出血性大肠杆菌(EHEC)O157∶H7主要保护性抗原紧密素和志贺毒素的融合蛋白。
The McAbs only reacted with EHEC O157 ∶ H7 but not with the other E. coli without intimin and Shiga-toxin. 应用3株单抗均可特异性检出EHECO157∶H7,而3株单抗与其他不产生紧密素和志贺毒素的大肠杆菌不反应。